Journal: Cell & Bioscience
Article Title: PINK1 functions in mitophagy and mitochondrial homeostasis during mice oocyte maturation
doi: 10.1186/s13578-025-01460-4
Figure Lengend Snippet: Pink1 overexpression induces spindle assembly failure and chromosome misalignment in murine oocyte maturation. ( A ) Control (a) and Pink1 -OE (b-d) oocytes were stained with anti-a-tubulin antibody to visualize the spindle (green) and counterstained with PI (red) to observe chromosomes. Arrows indicate spindle defects and arrowheads indicate chromosome misalignment in MI oocytes. ( B ) Quantification of control and Pink1 -OE oocytes with spindle/chromosome defects (9.4 ± 0.75% [ n = 86] vs. 45.3 ± 1.10 [ n = 74], respectively). * p < 0.05 vs. control. Scale bars: 20 μm
Article Snippet: Rabbit monoclonal anti-γH2AX antibody (Cat#: ab81299), rabbit monoclonal anti-RAB8A antibody (Cat#: ab188574), mouse monoclonal anti-Myc antibody (Cat#: ab18185) were purchased from Abcam (Cambridge, UK); rabbit polyclonal anti-PINK1 antibody (Cat#: PA5-85243) was obtained from Thermo Fisher Scientific (Thermo Fisher Scientific, USA); mouse monoclonal- FITC-conjugated anti-α-Tubulin antibody (Cat#: F2168) was bought from Sigma (St. Louis, MO, USA); rabbit polyclonal antibodies anti-LC3 antibody (Cat#: 14600-1-AP), mouse-Monoclonal antibody (Cat#: 60004-1-Ig), rabbit polyclonal antibody anti-α-Tubulin antibody (Cat#: 11224-1-AP) were purchased from Proteintech (Wuhan, China); mouse monoclonal antibody anti-PRKN antibody (Cat#: sc-32282) was obtained from Santa Cruz Biotechnologies (Santa Cruz, CA).
Techniques: Over Expression, Control, Staining